Dose-dependent effect of continuous subcutaneous verapamil infusion on experimental acute pancreatitis in mice

摘要: Calcium antagonists may limit experimental tissue injury by membrane stabilization. We studied the effects of verapamil on pancreatic ultrastructure and zymogen extraction during diet-induced acute pancreatitis. Acute pancreatitis was induced in female Swiss-Webster mice feeding a choline- methionine-deficient diet (CD) supplemented with 1% ethionine (CDE). Varying doses normal saline were infused continuously at rate 0.5μl/hr for 96 hr through subcutaneously implanted osmotic pumps. The pancreata examined blindly light microscopy electron microscopy. Zymogen extracted from measured expressed per gram protein. Mean histological scores, calculated according to formula that incorporates extent necrosis, inflammation, acidophilia, edema, 14.1±4, 10.3±2, 9.9±4, 5.9±7, 12.5±4, 12.7±4 CDE-fed animals receiving 0, 0.14, 0.28, 0.56, 0.84, 1.12μM daily, respectively. Animals fed or CD had scores 0±0. Histological significantly lower treated 0.56μm compared who received no (p<0.05) associated reduced dissolution granule EM. trypsinogen chymotrypsinogen content CD- mice. reduction mean reached statistical significance 0.56μM daily. also CD-fed 0.84μM Increasing protect against This protection is dissolution.