La conservazione dell'Orso bruno ( Ursus arctos ) in Appennino: il supporto della genetica non invasiva

作者: Mario Posillico , Luciano Sammarone , Rita Lorenzini , Leonardo Gentile , Rosario Fico

DOI: 10.4404/HYSTRIX-15.2-4337

关键词:

摘要: Conservation of the brown bear Ursus arctosin Apennines: a non- invasive genetic approach. Molecular techniques were used to assess individual identifica- tion for 24 bears (Ursus arctos)from Apennine population through noninvasive sampling. Shed hairs collected and as source DNA derive single genoty- pe profiling at 12 microsatellite loci. Genotypes 11 obtained from tissues or blood dead caught animals. Average expected heterozygosity (H E = 0.432) mean

参考文章(47)
Christian Schlötterer, David B. Goldstein, Microsatellites: Evolution and Applications ,(1999)
M.E. SOULE (ed), Viable Populations for Conservation ,(1987)
James F. Crow, Motoo Kimura, THE NUMBER OF ALLELES THAT CAN BE MAINTAINED IN A FINITE POPULATION. Genetics. ,vol. 49, pp. 725- 738 ,(1964) , 10.1093/GENETICS/49.4.725
Matthew A. Cronin, Steven C. Amstrup, Gerald W. Garner, Ernest R. Vyse, Interspecific and intraspecific mitochondrial DNA variation in North American bears (Ursus) Canadian Journal of Zoology. ,vol. 69, pp. 2985- 2992 ,(1991) , 10.1139/Z91-421
Pierre Taberlet, Sally Griffin, Benoît Goossens, Sophie Questiau, Valérie Manceau, Nathalie Escaravage, Lisette P Waits, Jean Bouvet, Reliable Genotyping of Samples with Very Low DNA Quantities Using PCR Nucleic Acids Research. ,vol. 24, pp. 3189- 3194 ,(1996) , 10.1093/NAR/24.16.3189
J. M. PEMBERTON, J. SLATE, D. R. BANCROFT, J. A. BARRETT, Nonamplifying alleles at microsatellite loci: a caution for parentage and population studies. Molecular Ecology. ,vol. 4, pp. 249- 252 ,(1995) , 10.1111/J.1365-294X.1995.TB00214.X
P. TABERLET, J.-J. CAMARRA, S. GRIFFIN, E. UHRÈS, O. HANOTTE, L. P. WAITS, C. DUBOIS-PAGANON, T. BURKE, J. BOUVET, Noninvasive genetic tracking of the endangered Pyrenean brown bear population. Molecular Ecology. ,vol. 6, pp. 869- 876 ,(1997) , 10.1111/J.1365-294X.1997.TB00141.X
Michael H. Kohn, Eric C. York, Denise A. Kamradt, Gary Haught, Raymond M. Sauvajot, Robert K. Wayne, Estimating population size by genotyping faeces Proceedings of The Royal Society B: Biological Sciences. ,vol. 266, pp. 657- 663 ,(1999) , 10.1098/RSPB.1999.0686