Corneal glycosaminoglycan synthesis in long-term organ culture.

摘要: types. Physical or enzymatic damage to the cultured corneas resulted in marked changes ratio of labeled GAG types secreted. epithelial endothelial surfaces and/or mild trypsin treatment before culture a stimulation galactosaminoglycan secretion and consequent decrease relative abundance keratan sulfate. The presence soy inhibitor prevented these changes. Treatment with collagenase all secretion, particularly When were removed from chilled, enucleated eyes rinsed solutions incubation, secreted had similar sulfate galactosaminglycan as vivo. This was maintained 1 week culture. These results suggest that characteristic connective tissue corneal stroma by keratocytes is dependent on interaction between cells components stromal extracellular matrix. Invest Ophthalmol Vis Sci 24:208-213, 1983 In adult animals, keratocytes, flattened mesenchymal located lamellae. For short periods time organ culture, continue secrete components. After 48 hours cell take place nature secretions. A well-characterized aspect this change alteration glycosaminglycan synthesis. There are two major glycosaminoglycan (GAG), (a chondroitin sulfate-dermatan hybrid). vivo, more than half total sulfate, but after level decreases 10% less total. 23 Keratocytes grown