Ischemic myocardial injury in cultured heart cells: preliminary observations on morphology and beating activity.

摘要: An in vitro model of myocardial ischemia has been established with primary monolayer cultures neonatal rat heart cells. Ischemic conditions were simulated by subjecting the cell to various levels oxygen and glucose deprivation. After ischemic treatments, beating muscle (M) cells evaluated for functional morphological changes. The experimental protocol consisted treatment 20% or 0% O2 1000, 500 0 mg per 1 medium 4, 12 24 hr. Control treated 1000 glucose. alterations induced deficiency formation pseudopodia cytoplasmic vacuoles; increased granulation; abnormal shapes, such as long, spindly shaped M There was a time-dependent decrease activity exposed longer durations conditions. However, if replenished complete (1000 glucose) O2, regained their ability beat.