Differing reactions of monoclonal anti-A antibodies with oligosaccharides related to blood group A.

摘要: Abstract Inhibition radioimmunoassays with blood group A-related oligosaccharides have been used to investigate the specificities of six monoclonal anti-A antibodies, three which had intentionally generated by immunization mice A erythrocytes and A-active substance, were incidentally produced following human tonsil cell membranes or a colon cancer line. By hemagglutination, these antibodies are highly specific for erythrocytes. However, they differ from one another in their reaction patterns mono- difucosyl antigen structures corresponding afucosyl sequences on Type 1 2 backbone structures. The together four previously characterized (originally raised against receptor epidermal growth factor) classified into five groups. first two groups consist broad There (TL5, 29.1, A17/3D1, MH2/6D4, MH1/5D1) reacting varying degrees either type sequence. In second (A15/3D4 A15/3D3) difficult inhibit tested, but reacted best monofucosyl structure backbone. Each remaining distinct more restricted pattern, specificity both types (antibody EGR/G49) 1-based MAS 016c) 2-based 455). reactions N-acetylgalactosamine containing sequence GalNAc alpha 1-3Gal suggest that may react certain glycoconjugates alpha-N-acetylgalactosaminyl termini ("A-like" structures) unrelated products gene-specified alpha-N-acetylgalactosaminyl-transferase. Knowledge differing is important interpreting glycoproteins glycolipids diverse origins.