Distribution of cannabinoid receptor 1 in the CNS of zebrafish.

摘要: The cannabinoid receptor 1 (Cb1) mediates the psychoactive effect of marijuana. In mammals, there is abundant evidence advocating importance signaling; activation Cb1 exerts diverse functions, chiefly by its ability to modulate neurotransmission. Thus, much attention has been devoted understand role in health and disease evaluate therapeutic potential. Here, we have cloned zebrafish cb1 investigated expression developing adult brain. Sequence analysis showed that a high degree conservation, especially residues demonstrated be critical for function mammals. situ hybridization revealed appears first preoptic area at 24 hours post-fertilization. Subsequently, transcripts are detected dorsal telencephalon, hypothalamus, pretectum torus longitudinalis. A similar pattern recapitulated While intensively stained medial zone elsewhere weak comparison. particular, localization telencephalic periventricular matrix suggestive involvement neurogenesis, bearing strong resemblance terms proliferative mammalian hippocampal formation. addition, gradient-like longitudinalis, teleost specific neural tissue. relation dopaminergic neurons diencephalic posterior tuberculum (considered teleostean homologue midbrain system), both tyrosine hydroxylase-expressing cells occupy non-overlapping domains. However they co-localized caudal implying direct modulation dopamine release this particular region. Collectively, our data indicate propensity participate multiple neurological processes.