Efficient production of doubled haploid wheat plants by in vitro treatment of microspores with trifluralin or APM
作者： N. J. P. Hansen , S. B. Andersen
DOI: 10.1111/J.1439-0523.1998.TB01963.X
关键词:
摘要: Isolated microspore cultures from two doubled haploid (DH) lines of wheat, Triticum aestivum L., were used to develop an in vitro chromosome-doubling protocol. During the initial 24h or 48h culture microspores treated with either antimicrotubule herbicides trifluralin amiprophos-methyl (APM) concentrations ranging 0.1 μM 10 μM. Untreated control yielded 209 embryos per 100 000 microspores, which is equivalent one spike. Among regenerated plantlets 67% green, and 15% flowering plants spontaneously chromosome doubled. Treatments both had a significant effect on doubling, measured as percentage fertile regenerants. With best combination treatment duration (48 h) herbicide concentration (10 μM) among regenerants could be increased up 74% APM 65% trifluralin. The largest numbers DH spike obtained at 1-3 these resulted estimated average between genotypes 27 microspores. These results demonstrate high potential agents isolated cultures. integrated into tissue procedures will constitute efficient method for doubling future wheat breeding.
sci-hub.se 参考文章(33)
N.J.P. Hansen, S.B. Andersen, In vitro chromosome doubling with colchicine during microspore culture in wheat (Triticum aestivum L.) Euphytica. ,vol. 102, pp. 101- 108 ,(1998) , 10.1023/A:1018348816205
A.L. Hansen, A. Gertz, M. Joersbo, S.B. Andersen, Antimicrotubule herbicides for in vitro chromosome doubling in Beta vulgaris L. ovule culture Euphytica. ,vol. 101, pp. 231- 237 ,(1998) , 10.1023/A:1018380103304
C. M�llers, M. C. M. Iqbal, G. R�bbelen, Efficient production of doubled haploid Brassica napus plants by colchicine treatment of microspores Euphytica. ,vol. 75, pp. 95- 104 ,(1994) , 10.1007/BF00024536
FX Oury, M. Pichon, M. Rousset, J. Gourdon, F. Lagoutte, Une comparaison entre 2 méthodes d'haplodiploïdisation chez le blé tendre : l'androgenèse in vitro et le croisement interspécifique avec le maïs Agronomie. ,vol. 13, pp. 95- 103 ,(1993) , 10.1051/AGRO:19930203
F.L. Hansen, S.B. Andersen, I.K. Due, A. Olesen, Nitrous oxide as a possible alternative agent for chromosome doubling of wheat haploids Plant Science. ,vol. 54, pp. 219- 222 ,(1988) , 10.1016/0168-9452(88)90116-1
Marcia M. Falconer, R. W. Seagull, Amiprophos-methyl (APM): a rapid, reversible, anti-microtuble agent for plant cell cultures Protoplasma. ,vol. 136, pp. 118- 124 ,(1987) , 10.1007/BF01276360
K. Sree Ramulu, H. A. Verhoeven, P. Dijkhuis, Mitotic blocking, micronucleation, and chromosome doubling by oryzalin, amiprophos-methyl, and colchicine in potato Protoplasma. ,vol. 160, pp. 65- 71 ,(1991) , 10.1007/BF01539957
StephenJ. Mejza, Vincent Morgant, DeniseE. DiBona, JamesR. Wong, Plant regeneration from isolated microspores of Triticum aestivum. Plant Cell Reports. ,vol. 12, pp. 149- 153 ,(1993) , 10.1007/BF00239096
Jiping Zhao, Daina H. Simmonds, Application of trifluralin to embryogenic microspore cultures to generate doubled haploid plants in Brassica napus Physiologia Plantarum. ,vol. 95, pp. 304- 309 ,(1995) , 10.1111/J.1399-3054.1995.TB00842.X
Shuang-E Jia, Chi Zhang, Tie-Han Zhao, Li-Zhen He, Xu Jia, Jun-Wen Ouyang, Hui Liang, Studies on the chromosome doubling of wheat pollen plants Plant Science. ,vol. 98, pp. 209- 214 ,(1994) , 10.1016/0168-9452(94)90011-6