Positive Selection Drives Preferred Segment Combinations during Influenza Virus Reassortment

摘要: Influenza A virus (IAV) has a segmented genome that allows for the exchange of segments between different strains. This reassortment accelerates evolution by breaking linkage, helping IAV cross species barriers to potentially create highly virulent Challenges associated with monitoring process in molecular detail have limited our understanding its evolutionary implications. We applied novel deep sequencing approach quantitative analysis assess vitro temporal genomic IAV. The combination H1N1 and H3N2 strains reproducibly generated new H1N2 strain hemagglutinin nucleoprotein originating from remaining six H3N2. By entire viral genome, we monitored reassortment, quantifying relative abundance all two parent over time measuring selection coefficients reassorting segments. Additionally, observed several mutations coemerging were not found during passaging pure parental Our results demonstrate how can accelerate IAV, enabled emergence small number individual mutations.