MiR-23a in amplified 19p13.13 loci targets metallothionein 2A and promotes growth in gastric cancer cells

摘要: Copy number variation (CNV) and abnormal expression of microRNAs (miRNAs) always lead to deregulation genes in cancer, including gastric cancer (GC). However, little is known about how CNVs affect the miRNAs. By integrating CNV miRNA profiles same samples, we identified eight miRNAs (miR-1274a, miR-196b, miR-4298, miR-181c, miR-181d, miR-23a, miR-27a miR-24-2) that were located amplified regions upregulated GC. In particular, amplification miR-23a-27a-24-2 cluster miR-181c-181d frequently occurred at 19p13.13 confirmed by genomic real-time PCR another 25 paired GC samples. Moreover, situ hybridization (ISH) experiments represented mature miR-23a was increased GCs (75.5%, 40/53) compared with matched normal tissues (28.6%, 14/49, P = 0.001). Knocking down inhibited BGC823 cell growth vitro vivo. addition, potential target investigated integration mRNA profile TargetScan predictions, found upregulation downregulation metallothionein 2A (MT2A) detected simultaneously 70% (7/10) profiles. Furthermore, an inverse correlation between MT2A tissues. Through combining luciferase assay, a miR-23a. conclusion, these results suggest CNV-miRNA-mRNA profiling powerful tool for identifying molecular signatures, might play role regulating J. Cell. Biochem. 114: 2160–2169, 2013. © 2013 Wiley Periodicals, Inc.