Hepatocyte transport of bile acids and organic anions in endotoxemic rats : impaired uptake and secretion

摘要: BACKGROUND & AIMS: In sepsis, intrahepatic cholestasis occurs frequently, suggesting impaired hepatocyte transport of bile acids and organic anions. The aim the study was to define magnitude, time course, site biliary secretion in a rat sepsis model. METHODS: Maximal for two (cholyltaurine chenodeoxycholyltaurine) anions (sulfobromophthalein sulfolithocholyltaurine) measured isolated perfused livers at various times after lipopolysaccharide injection. Basolateral canalicular liver plasma membrane vesicles were used characterize impairment transport. RESULTS: reduced all compounds by 60%-81% compared with controls. Bile acid-independent flow 51%. Impairment maximal 12 hours endotoxin injection recovered thereafter. basolateral vesicles, sodium-dependent 36%-47%. Sodium-independent 40%-55%. Adenosine triphosphate-stimulated greatly decreased prepared from endotoxemic animals four probably because number molecules, based on kinetic studies. CONCLUSIONS: acid anion are markedly endotoxemia. These mechanisms may contribute sepsis. (Gastroenterology 1997 Jan;112(1):214-25)