Nuclear magnetic resonance spectroscopy for studying the development and detection of the grain weevil, Sitophilus granarius (L.) (Coleoptera: Curculionidae), within wheat kernels

摘要: A novel non-destructive method was devised for studying the development of insects hidden within individual wheat grains using a standard nuclear magnetic resonance (NMR) spectrometer. The Sitophilus granarius (L.) at 25°C and 70% RH studied from egg-laying to emergence by NMR, X-radiography weighing. results three methods correlated quite well. Neither NMR nor X-radiation appeared harm insects. did not usually detect until they were in third larval instars, but after that areas two peaks observed with each infested grain followed pattern which readily interpreted terms insect development. major peak found be due almost wholly water minor lipid, size obtained adults close expected on basis their content. movement some short periods characteristic developmental stage. Preliminary work less sophisticated machine showed it is possible single scan ten S. larvae batch 500 grains, further required see whether eggs can detected. this study sufficiently encouraging wide variety other suggested use might help solve entomological problems.