Analysis of CD8+ Treg cells in patients with ovarian cancer: a possible mechanism for immune impairment.

摘要: Regulatory T (Treg) cells may participate in mediating a suppressive microenvironment that blunts successful anti-tumor immunotherapy. Recent studies show CD8+ Treg might impede effective immune responses to established tumors. However, there is limited research regarding ovarian cancer (OC) patients. Here, we investigated OC patients and their vitro induction. The immunohistochemistry of tumor-infiltrating lymphocytes revealed significant correlation between the intratumoral forkhead box p3 (Foxp3)+ intraepithelial stromal areas advanced tissues. We examined expression markers from peripheral blood fresh tumor tissues using flow cytometry. Our results indicated an increase cell subsets compared with those benign tumors healthy controls, including increased CD25, cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), Foxp3 decreased CD28 expression. To demonstrate whether could convert effector into suppressor cells, used transwell culturing system. Compared cultured alone, induced by coculture SK-OV-3/A2780 showed CTLA-4 In addition, vitro-induced inhibited naive CD4+ T-cell proliferation, which was partially mediated through TGF-β1 IFN-γ. study suggests were be vitro, way limit antitumor immunity evade surveillance.