Telomerase regulation during entry into the cell cycle in normal human T cells.

摘要: Telomerase activity is involved in telomere length maintenance. Leukocytes, unlike many human somatic tissues, have detectable telomerase activity. These cells provide a normal cell type which to study telomerase. We studied the regulation of and RNA component as leukocytes were stimulated enter cycle. In primary with phytohemagglutinin, increased > 10-fold naturally quiescent entered Antibodies T receptor (TCR)/CD3 complex costimulatory CD28 induced cell-enriched population cells. Rapamycin, an immunosuppressant that blocks TCR/CD3 signal transduction pathways cdk2 activation, blocked induction. Hydroxyurea, inhibitor S phase, did not block kinase or activation. summary, regulated G1 phase