The Dorsal Column Lesion Model of Spinal Cord Injury and Its Use in Deciphering the Neuron-Intrinsic Injury Response

摘要: The neuron-intrinsic response to axonal injury differs markedly between neurons of the peripheral and central nervous system. Following a lesion, robust growth program is initiated, whereas system do not mount an effective regenerative response. Increasing would therefore be one way promote regeneration in injured large-diameter sensory located dorsal root ganglia are pseudo-unipolar that project axon branch into spinal cord, and, via column brain stem, process muscles skin. Dorsal ganglion ideally suited study because they exhibit successful following axotomy, while fail so after lesion column. model allows studied context cord injury. Here we will discuss advantages disadvantages this model. We describe surgical methods used implement ascending fibers, anatomy afferent pathways anatomical, electrophysiological, behavioral techniques quantify functional recovery. Subsequently review results experimental interventions model, with emphasis on molecular mechanisms govern manipulations these axotomy. Finally, highlight number recent advances have impact design future studies including continued development adeno-associated viral vectors likely improve genetic manipulation use tissue clearing enabling 3D reconstruction regenerating tracts. © 2018 Authors. Developmental Neurobiology Published by Wiley Periodicals, Inc. Develop Neurobiol 00: 000-000, 2018.