Receptor-specific agglutination tests for detection of bacteria that bind globoseries glycolipids.

摘要: Specific binding to the globoseries of glycolipid receptors explains adherence uropathogenic Escherichia coli host cells. The minimal receptor disaccharide Gal alpha 1----4Gal beta [galactose (1----4)galactose beta] is recognized by most attaching clinical isolates. However, wild-type isolates can express adhesins with several different specificities. Bioassays do not permit separate analysis each specificity, since target cells contain multiple potentially receptor-active molecules. In this study, bacterial were analyzed using immobilized into latex beads in one two ways. way, di- and trisaccharides covalently linked via a spacer arm coupled bovine serum albumin. other glycolipids coated onto albumin-latex beads. subsequently used for agglutination type strains known specificity. composition was optimized regarding structure size as active trisaccharide derivative 1----3glucose or 1----3glucosamine. Among natural tested, globotetraosylceramide active. Subsequently, sensitivity specificity beta-latex globotetraosylceramide-latex reagents compared 733 E. urinary Hemagglutination human erythrocytes positive standard. No significant difference found; ranged from 86%, when agglutinating blood groups P1 p included, 93%, those group excluded. These provide tools identification patients tract infection.