Targeted profiling of atherogenic phospholipids in human plasma and lipoproteins of hyperlipidemic patients using MALDI-QIT-TOF-MS/MS

摘要: Abstract Objectives Phospholipids (PLs) are increasingly recognized as key molecules with potential diagnostic value in acute inflammation, CVD and atherosclerosis. We introduce a pioneer mass spectrometry (MS)-based approach aiming to investigate the relationship of specific plasma PL-subsets atherogenic blood parameters young patients familial hyperlipidemia representing high-CVD-risk groups. Methods Plasma carefully phenotyped FH FCH well normolipidemic subjects (age 13 ± 5 years, n  = 20) was used. Clinical were assessed using standard laboratory techniques lipids subjected direct targeted monitoring LC-ESI-SRM- MALDI-QIT-TOF-MS/MS, respectively. Statistical analysis performed evaluate correlations between PL data clinical parameters. Results Most characteristically significant differences SM/PC PC/LPC ratios positive SM vs. LDL-C ( r  = 0.946; p  = 0.004) LPC VLDL-C  = 0.669;  = 0.218) were observed contrast other study OxPC levels found range ∼2–20 μmol/L predominance short-chain aldehydic species (e.g. SOVPC). A correlation OxPCs IMT  = 0.952;  = 0.052) HDL-C  = 0.893;  = 0.016) but negative correlation with OxLDL  = −0.910;  = 0.096) observed. Conclusions Our first attempt use MALDI-QIT-TOF-MS/MS based lipidomics dyslipidemia hyperlipidemia. This technique represents promising platform for screening lipid biomarkers future.