Characterization of stress degradation products of benazepril by using sophisticated hyphenated techniques.

摘要: Benazepril, an anti-hypertensive drug, was subjected to forced degradation studies. The drug unstable under hydrolytic conditions, yielding benazeprilat, which is a known major product (DP) and active metabolite. It also underwent photochemical in acid neutral pH resulting multiple minor DPs. products were separated on reversed phase (C18) column gradient mode, LC–MS LC–NMR Initially, comprehensive mass fragmentation pathway of the established through support high resolution spectrometric (HR-MS) multi stage tandem (MSn) data. DPs LC–MS/TOF studies obtain their accurate masses. Along with, on-line H/D exchange data obtained ascertain number exchangeable hydrogens each molecule. LC–1H NMR LC–2DNMR additionally acquired fraction loop mode. whole information successfully employed for characterization all A complete established.