Establishing the Framework for Fabrication of a Bioartificial Heart.

摘要: There is a chronic shortage of donor hearts. The ability to fabricate complete bioartificial hearts (BAHs) may be an alternative solution. current study describes method support the fabrication and culture BAHs. Rat were isolated subjected detergent based decellularization protocol remove all cellular components, leaving behind intact extracellular matrix. Primary cardiac cells from neonatal rat hearts, direct cell transplantation was used populate acellular scaffolds. Bioartificial maintained in custom gravity fed perfusion system media delivery. functional performance BAHs assessed on left ventricle pressure electrocardiogram. Furthermore, sectioned stained for whole heart tissue distribution molecules, such as α-actinin, troponin I, collagen type connexin 43, von Willebrand factor, ki67. replicated partial subset properties natural provided BAH revealed challenges toward with metrics mammalian