Enrichment of neonatal rat cardiomyocytes in primary culture facilitates long-term maintenance of contractility in vitro.

摘要: Long-term culture of primary neonatal rat cardiomyocytes is limited by the loss spontaneous contractile phenotype within weeks in culture. This may be due to from or overgrowth non-cardiomyocyte population. Using mitochondria specific fluorescent dye, tetramethylrhodamine methyl ester perchlorate (TMRM), we showed that enriched fluorescence-activated cell sorting can maintained as cultures for long periods (24-wk vs. 2 wk unsorted cardiomyocytes). this purified cardiomyocyte (TMRM high) population retained expression markers, continued calcium cycling, and displayed cyclic electrical activity could regulated pharmacologically. These findings suggest populations negatively influence contractility purifying cardiomyocytes, retain potential an experimental model longitudinal studies biology vitro.