Three-dimensional reconstruction: a tissue embedding method for alignment of serial sections
作者： Eric P. Meyer , Valerie J. Domanico
DOI: 10.1016/0165-0270(88)90162-8
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摘要: Three-dimensional (3D) reconstruction from serial sections that are undistorted and true-to-life is only possible when a reference system exists allows correct matching of the section drawings. This paper describes simple method for producing lines ensures proper alignment (1) The specimen embedded in rectangular form, (2) sides block painted, (3) sectioned exactly perpendicular to painted block. Thus, each paint appears as dark which serve sections.
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