Transendothelial migration drives dissociation of plateletmonocyte complexes

摘要: Monocytes and platelets are both crucially involved in atherogenesis. Importantly, activated bound to circulating monocytes increase adhesion of the thus mediate colocalization cell types at vessel wall. We examined fate upon migration these potentially pro-atherogenic platelet-monocyte complexes (PMC) across endothelium. Platelet-monocyte complex was studied quantitatively by means Transwell filters coated with endothelial cells, as well qualitatively different imaging techniques, absence or presence flow. Upon PMC transendothelial migration, relocate monocytic P-selectin glycoprotein ligand-1 (PSGL-1) rear monocyte, detach, remain surface. Platelet dissociation appeared not be due reduced PSGL-1 expression platelet-binding capacity migrated monocytes. In addition, matrix proteins capacities on filter, instead did affect dissociation. contrast, lowering mechanical stress that experience during transmigration prevented platelets. conclusion, dissociate a result redistribution stress. PMC-mediated deposition sites vascular inflammation is likely relevant for cardiovascular disease progression regeneration. See also following online supplementary material: Video 1Video 2