Platelet binding to monocytes increases the adhesive properties of monocytes by up-regulating the expression and functionality of β1 and β2 integrins

摘要: Human monocytes adhere to activated platelets, resulting in the formation of platelet-monocyte complexes (PMC). Complex depends on interaction between platelet-displayed P-selectin and specific ligand for leukocytes, glycoprotein ligand-1 (PSGL-1). We have recently shown that within PMC increased adhesive capacity endothelium. To better understand effect platelet binding endothelium, P-selectin-PSGL-1 interaction-induced changes integrin functionality were studied. The platelets via interactions was increase expression activity alpha4beta1 alphaMbeta2integrin, with a concomitant decrease L-selectin expression. Furthermore, resulted monocyte adhesion intercellular molecule-1, vascular cell fibronectin. Platelet also responsible an transendothelial migration. Similar effects observed after engagement PSGL-1 antibodies or immunoglobulin protein. Our data suggest by monocytes, induce up-regulation activation beta1 beta2integrins Hence, are higher state may have, therefore, atherogenic capacity.