Impact of anemia prevention by recombinant human erythropoietin on the sensitivity of xenografted glioblastomas to fractionated irradiation.

摘要: Pronounced oxygen deficiency in tumors which might be caused by a diminished transport capacity of the blood (e. g., anemia) reduces efficacy ionizing radiation. The aim this study was to analyze whether anemia prevention by recombinant human erythropoietin (rHuEPO) affects the radiosensitivity glioblastoma xenografts during fractionated irradiation. Anemia induced total body irradiation (TBI, 2 × 4 Gy) mice prior tumor implantation into subcutis the hind leg. In one experimental group, development anemia was prevented rHuEPO (750 U/kg s.c.) given three times weekly starting 10 days TBI. 13 after implantation (tumor volume approx. 40 mm3), fractionated (4 7 Gy, daily fraction) the glioblastomas performed resulting growth delay with subsequent regrowth tumors. Compared nonanemic control animals (hemoglobin concentration cHb = 14.7 g/dl), anemic mice (cHb 9.9 g/dl) significantly shorter (49 ± 5 vs. 79 ± 4 reach four initial volume) upon fractionated radiation. The prevention rHuEPO treatment (cHb 13.3 resulted significantly prolonged (61 days) compared anemia group, even though inhibition found control animals not completely achieved. These data indicate that moderate significantly reduces radiotherapy. Prevention with rHuEPO partially restores radiosensitivity xenografted glioblastomas fractionated