The interfering effects of superovulation and vitrification upon some important epigenetic biomarkers in mouse blastocyst.
作者： Azizollah Bakhtari , Hamid-Reza Rahmani , Elham Bonakdar , Farnoosh Jafarpour , Vajiheh Asgari
DOI: 10.1016/J.CRYOBIOL.2014.09.379
关键词:
摘要: Appropriate epigenetic changes in preimplantation embryos are critical for embryonic development and successful pregnancy. The aim of this study was to evaluate the effects some assisted reproductive techniques (ARTs) on a panel biomarkers by immunofluorescence staining at blastocyst stage. For purpose, four treatment groups were designed: control (C), superovulation (S), superovulation+in vitro culture (SI), superovulation+vitrification+in (SVI). Results showed that vitrification decreased developmental competence cultured (P<0.05). Semi-quantitative analysis revealed fluorescence intensity global DNA methylation inner cell mass (ICM), SVI Group comparison C group Superovulation, elevated level H3K9acetylation trophectoderm (TE) SI Furthermore, ARTs manipulations influenced ICM H4K12acetylation TE higher than S H3K4tri-methylation, had Finally, Pou5f1 protein signal vivo-derived stage In conclusion, ART may have important influences multiple biomarkers.
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