Quantitative detection of allergenic protein Sin a 1 from yellow mustard (Sinapis alba L.) seeds using enzyme-linked immunosorbent assay.
作者： Youn-Young Shim , Janitha P. D. Wanasundara
DOI: 10.1021/JF072660U
关键词:
摘要: Allergy to yellow mustard (YM; Sinapis alba L.) seed proteins has been reported and is currently seen as a constraint that hampers expansion of YM protein utilization. The most predominant allergenic recognized Sin 1. In this study, 1 was purified (S. var. Andante), rabbit polyclonal antibodies (pAb) specific were generated, sandwich enzyme-linked immunosorbent assay (S-ELISA) developed detect quantify from YM. S-ELISA method using 1−pAb its horseradish peroxidase conjugate resulted in detection limit 0.3 µg/mL for contents six lines the range 0.82–2.94 mg/g when assayed by method. results showed could distinguish seed-derived extracts rapidly be applied controlling and/or monitoring proteins.
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