Sample preparation for MALDI mass analysis of peptides and proteins.

摘要: Sample preparation may be the most crucial step in mass spectrometric analysis of peptides and proteins by MALDI. The free dipolar ionic or must incorporated into a crystalline solid solution that is comprised excess solvent (the matrix), which modulates desorption/ionization preferentially absorbing laser energy at irradiation wavelength pulse intensity being employed. Usually cocrystalline sample matrix are prepared mixing solutions each component permitting mixture to crystallize through evaporation solvents. Two methods employing this approach presented unit: dried drop method rapid method.