pH-Dependent Entry of Severe Acute Respiratory Syndrome Coronavirus Is Mediated by the Spike Glycoprotein and Enhanced by Dendritic Cell Transfer through DC-SIGN

摘要: The severe acute respiratory syndrome coronavirus (SARS-CoV) synthesizes several putative viral envelope proteins, including the spike (S), membrane (M), and small (E) glycoproteins. Although these proteins likely are essential for replication, their specific roles in SARS-CoV entry have not been defined. In this report, we show that S glycoprotein mediates through pH-dependent endocytosis. Further, define its cellular tropism demonstrate virus transmission occurs cell-mediated transfer by dendritic cells. was used successfully to pseudotype replication-defective retroviral lentiviral vectors readily infected Vero cells as well primary pulmonary renal epithelial from human, nonhuman primate, and, a lesser extent, feline species. of reporter similar wild-type, replication-competent SARS-CoV, binding purified susceptible target demonstrated flow cytometry. myeloid were able interact with bind virus, could be SARS-CoV. However, synapse-like structure. Both infection direct inhibited anti-S antisera, indicating strategies directed toward gene product confer therapeutic benefit antiviral drugs or development SARS vaccine.