Characterization of syngeneic antiidiotypic monoclonal antibodies to murine anti-human high molecular weight melanoma-associated antigen monoclonal antibodies.

摘要: Hybridization with murine myeloma cells P3-X63-Ag8.653 of splenocytes from BALB/c mice immunized syngeneic anti-human high molecular weight melanoma-associated Ag (HMW-MAA) mAb 149.53, 225.28, 763.74, and TP41.2 has resulted in the formation antiidiotypic antibody-secreting hybridomas a frequency ranging between 1.2% 5.2%. No marked difference was detected antibody secreting fusions generated four anti-HMW-MAA mAb, suggesting that idiotopes expressed by each them display similar immunogenicity combination. The number did not inhibit binding immunizing to melanoma higher than those died, associated Ag-combining site are more immunogenic are. recognized were on large panel anti-HLA Class I II mAb. latter included also cross-inhibit isolated H L chain Cross-blocking experiments selected identified three distinct two 763.74. Three, 5, 2, 5 within TP41.2, respectively, tested for their ability induce antibodies. Serological immunochemical assays antibodies only sera MK2-23. Therefore, MK2-23 can be classified as beta, while remaining 14 gamma.