作者: Yangyang Wang , Francesco Sabbatino , Xinhui Wang , Soldano Ferrone
DOI: 10.1007/978-1-62703-727-3_28
关键词: Antibody 、 Cancer research 、 Tumor antigen 、 CSPG4 、 Antigen 、 Melanoma 、 Immunotherapy 、 Chondroitin Sulfate Proteoglycan 4 、 Immunohistochemistry 、 Chemistry
摘要: The tumor antigen chondroitin sulfate proteoglycan 4 (CSPG4) appears to be a useful biomarker identify melanoma cells and an attractive target apply antibody-based immunotherapy for the treatment of melanoma. Here we described reverse transcription-polymerase chain reaction (RT-PCR) method immunohistochemical (IHC) staining detect expression CSPG4 in tissues.