Breast Tumor-Initiating Cells Isolated from Patient Core Biopsies for Study of Hormone Action

摘要: In recent years, evidence has emerged supporting the hypothesis that cancer is a stem cell disease. The field was led by discovery of leukemia cells (Tan, B.T., Park, C.Y., Ailles, L.E., and Weissman, I.L. (2006) hypothesis: work in progress. Laboratory Investigation. 86, 1203-1207), within past few years have been isolated from number solid tumor including those breast brain among others (Al-Hajj M., Wicha M.S., Benito-Hernandez A., Morrison, S.J., Clarke, M.F. (2003) Prospective identification tumorigenic cells. Proc. Natl. Acad. Sci. USA 100, 3983-3988; Singh, S.K., I.D., Terasaki, Bonn, V.E., Hawkins, C., Squire, J., Dirks, P.B. Identification Cancer Stem Cell Human Brain Tumors. Research. 63, 5821-5828). exhibit far different properties than established lines such as relative quiescence, multidrug resistance, multipotency (Clarke, M.F., Dick, J.E., P.B., Eaves, C.J., Jamieson, C.H.M., Jones, D.L., Visvader, I.L., Wahl, G.M. Cells-Perspectives on Current Status Future Directions: AACR Workshop Cells. 66, 9339-9344). addition, our laboratory demonstrated strong metastatic phenotype when passaged mice. Since these somewhat unique properties, it will be important for endocrinologists to evaluate hormonal action precursor more thorough understanding biology development effective treatment modalities. A relatively easy low cost method developed isolate primary needle biopsies taken patients diagnosed with invasive ductal carcinoma during routine care consent IRB approval. Fresh (2-3 at 2 cm length) were enzymatically dissociated collagenase (300 U/ml)/hyaluronidase (100 U/ml) solution followed sequential filtration. Single suspensions cultured ultra attachment plastic flasks defined medium formed non-adherent tumorspheres. tumorspheres exhibited surface marker expression CD44(+)/CD24(low/-)/ESA(+), previously "breast cell" Al Hajj et al.