Label-free detection of chondroitin sulphate proteoglycan 4 by a polyaniline/graphene nanocomposite functionalized impedimetric immunosensor

摘要: The chondroitin sulphate proteoglycan 4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMWMAA), is a tumor-associated that expressed in more than 85% of surgically removed melanoma lesions but has restricted distribution normal tissues. diagnostic and therapeutic value CSPG4 drives need for sensitive low-cost detection approaches. To this end, we developed polyaniline/graphene oxide nanocomposite ([email protected]) was electrochemically codeposited on indium tin (ITO) electrode. Glutaraldehyde mediated the covalent immobilization specific antibody mAbD2.8.5 to construct immunosensor using cell culture media lysate samples. fully assembled impedimetric used detect CSPG4-positive lines M14/CSPG4 MV3. No impedance signal changes could be observed from CSPG4-negative M14 mAbMk2-23 showing specificity CSPG4-impedimetric immunosensor. This low-cost, simple, label-free analytical method an alternative enzyme-linked immunosorbent assay flow cytometry screening complex biological