Nerve Guidance by a Decellularized Fibroblast Extracellular Matrix

摘要: Spinal cord and peripheral nerve injuries require the regeneration of fibers across lesion site for successful recovery. Providing guidance cues soluble factors to promote neurite outgrowth cell survival can enhance repair. The extracellular matrix (ECM) plays a key role in tissue repair by controlling adhesion, motility, growth. In this study, we explored ability mesenchymal ECM support from neurons superior cervical ganglia (SCG). Length morphology neurites extended on decellularized fibroblast were compared those substrates coated with laminin, major protein neural tissue, or fibronectin, main component ECM. Average radial extension was equivalent laminin ECM, but contrasted shorter, curved observed fibronectin substrate. Differences between other confirmed fast Fourier transform analyses. To control direction outgrowth, developed an linearly aligned fibril organization orienting fibroblasts that deposit polymeric surface micropatterned striped chemical interface. Neurites projected SCGs appeared reorient pattern. These results highlight directional neurites. This architecture has potential as regenerative microenvironment