Substratum preferences of motor and sensory neurons in postnatal and adult rats.

摘要: After peripheral nerve injuries, damaged axons can regenerate but functional recovery is limited by the specific reinnervation of targets. In this study we evaluated if motor and sensory neurites have a substrate preference for laminin fibronectin in postnatal adult stages. dorsal root ganglia (DRG) explants, neurons extended longer on collagen matrices enriched with (~50%) or (~35%), whereas motoneurons (~100%) organotypic spinal cord slices embedded fibronectin-enriched matrix. An increased percentage parvalbumin-positive (presumptive proprioceptive) vs. neurofilament-positive was also found DRG To test different extracellular matrix components maintained vivo, these were used to fill chitosan guide repair 6-mm gap sciatic rats. However, number regenerating after 1 month similar between groups. Moreover, none retrotraced positive parvalbumin, suggesting that presumptive proprioceptive had poor regenerative capabilities compared other neurons. Using real-time PCR expression α5β1 (receptor fibronectin) α7β1 integrin laminin) 2 days injury. Postnatal animals showed higher increase integrin, both integrins similarly expressed Therefore, conclude at early stages difference lost adult.