Neuregulin-1/glial growth factor stimulates Schwann cell migration by inducing α5 β1 integrin-ErbB2-focal adhesion kinase complex formation

摘要: After peripheral nerve injury, Schwann cells gain a migratory phenotype and remodel their extracellular matrix to provide supportive environment for axonal regeneration. The soluble neuregulin-1 isoform, that is, glial growth factor (GGF), is expressed in regenerating axons of injured nerves regulates cell motility by activating the ErbB family tyrosine kinase receptors, but how GGF/ErbB signaling contributes remains unclear. Here, we show GGF stimulates migration inducing formation protein complex containing fibronectin receptor α5β1 integrin, ErbB2, focal adhesion (FAK). ErbB2 co-localizes co-immunoprecipitates with members including integrin FAK after treatment. These effects appear involve activation, which occurs downstream stimulation. RNAi-mediated down-regulation α5 expression primary cultured resulted significantly decreased interaction between as well GGF-induced migration. An increase integrin-ErbB2-FAK was observed cells, not uninjured control. Taken together, these data suggest plays an important modulatory role crush formation.