Direct and Rapid Description of the Individual Ionic Currents of Squid Axon Membrane by Ramp Potential Control

摘要: Computations based upon the Hodgkin-Huxley equations and experimental data from squid axons show that ramp functions can be used as commands to a voltage clamp system selectively observe either fast (sodium) or slow (potassium) process in axon membranes without chemical separation techniques computer assistance. Each is characterized directly (on line) rapidly (real time) by generating current-potential curve on an oscilloscope for rates of change membrane potential (ramps). The speed directness this method characterizing each essential axonal events permit quantitative measurement kinetics rapid effects these processes due various pharmacological agents such tetrodotoxin tetraethylammonium ion other changes environment.