Stereo-Array Isotope Labeling Method for Studying Protein Structure and Dynamics
作者： Yohei Miyanoiri , Mitsuhiro Takeda , Masatsune Kainosho
DOI: 10.1007/978-94-007-4954-2_5
关键词:
摘要: The stereo-array isotope labeling (SAIL) method utilizes proteins with patterns optimized regard to an intended NMR study. SAIL are prepared by incorporating chemically synthesized amino acids into target proteins, using a cell-free protein synthesis system or cellular expression system. Over the past decade, has been facilitating wide variety of new investigations, including high-resolution structure determinations large and investigations dynamics. In this chapter, applications SAIL-related approaches introduced.
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Ronald A. Venters, Chih-Chin Huang, Bennett T. Farmer, Ronald Trolard, Leonard D. Spicer, Carol A. Fierke, High-level 2H/13C/15N labeling of proteins for NMR studies Journal of Biomolecular NMR. ,vol. 5, pp. 339- 344 ,(1995) , 10.1007/BF00182275
Peter Güntert, Automated NMR structure calculation with CYANA. Methods of Molecular Biology. ,vol. 278, pp. 353- 378 ,(2004) , 10.1385/1-59259-809-9:353
G Marius Clore, Angela M Gronenborn, Multidimensional heteronuclear nuclear magnetic resonance of proteins. Methods in Enzymology. ,vol. 239, pp. 349- 363 ,(1994) , 10.1016/S0076-6879(94)39013-4
Mitsuhiro Takeda, Nozomi Sugimori, Takuya Torizawa, Tsutomu Terauchi, Akira M. Ono, Hirokazu Yagi, Yoshiki Yamaguchi, Koichi Kato, Teppei Ikeya, JunGoo Jee, Peter Güntert, David J. Aceti, John L. Markley, Masatsune Kainosho, Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR. FEBS Journal. ,vol. 275, pp. 5873- 5884 ,(2008) , 10.1111/J.1742-4658.2008.06717.X
Ronald A. Venters, Bennett T. Farmer II, Carol A. Fierke, Leonard D. Spicer, Characterizing the use of perdeuteration in NMR studies of large proteins: 13C, 15N and 1H assignments of human carbonic anhydrase II. Journal of Molecular Biology. ,vol. 264, pp. 1101- 1116 ,(1996) , 10.1006/JMBI.1996.0699
Takuya Torizawa, Masato Shimizu, Masato Taoka, Hiroshi Miyano, Masatsune Kainosho, Efficient production of isotopically labeled proteins by cell-free synthesis: a practical protocol. Journal of Biomolecular NMR. ,vol. 30, pp. 311- 325 ,(2004) , 10.1007/S10858-004-3534-2
Mitsuhiro Takeda, JunGoo Jee, Akira Mei Ono, Tsutomu Terauchi, Masatsune Kainosho, Hydrogen exchange rate of tyrosine hydroxyl groups in proteins as studied by the deuterium isotope effect on Cζ chemical shifts Journal of the American Chemical Society. ,vol. 131, pp. 18556- 18562 ,(2009) , 10.1021/JA907911Y
Mitsuhiro Takeda, Chung-ke Chang, Teppei Ikeya, Peter Güntert, Yuan-hsiang Chang, Yen-lan Hsu, Tai-huang Huang, Masatsune Kainosho, Solution structure of the c-terminal dimerization domain of SARS coronavirus nucleocapsid protein solved by the SAIL-NMR method Journal of Molecular Biology. ,vol. 380, pp. 608- 622 ,(2008) , 10.1016/J.JMB.2007.11.093
Mitsuhiro Takeda, Tsutomu Terauchi, Masatsune Kainosho, Conformational analysis by quantitative NOE measurements of the β-proton pairs across individual disulfide bonds in proteins. Journal of Biomolecular NMR. ,vol. 52, pp. 127- 139 ,(2012) , 10.1007/S10858-011-9587-0
Stephan Grzesiek, Jacob Anglister, Hao Ren, Ad Bax, Carbon-13 line narrowing by deuterium decoupling in deuterium/carbon-13/nitrogen-15 enriched proteins. Application to triple resonance 4D J connectivity of sequential amides Journal of the American Chemical Society. ,vol. 115, pp. 4369- 4370 ,(1993) , 10.1021/JA00063A068