In vivo mutations at the H-2 locus in mouse lymphocytes

摘要: Abstract An experimental system has been developed to study in vivo autosomal mutations murine splenic lymphocytes. Mutant lymphocytes were isolated by immunocytotoxicity using monoclonal antibodies directed against the k and d alleles of K D H-2 histocompatibility loci enumerated limiting-dilution cloning. Genomic allele loss mutant clones was detected allele-specific primers a polymerase chain reaction. classified on basis phenotypic genotypic criteria into “no change”, deletion or recombination mutants. The geometric mean mutation frequency 102 mice 2.42 × 10−4. Detailed 87 from 4 revealed change” mutants 83%, due 7% 7%. Anomalous results obtained 3% clones. development an animal model for at locus will further advance mutations, particularly those involving chromosomal changes such as mitotic recombination.