The importin α subunit PsIMPA1 mediates the oxidative stress response and is required for the pathogenicity of Phytophthora sojae

摘要: Abstract The sensing of extracellular signals and their transduction into an appropriate response are crucial for the survival virulence plant pathogens. Eukaryotic pathogens must overcome obstacles posed by nuclear membranes to manipulate gene expression adapt host challenge. A highly sophisticated mechanism is use importins transport proteins nucleus. In this study, we identified a conserved importin α gene, PsIMPA1 , in Phytophthora sojae that was differentially expressed during life cycle soybean pathogen. lowest zoospores cysts but relatively consistent other stages, except slight increase at 6 h post infection. Silenced mutants Psimpa1 had decreased growth rate, aberrant mycelial morphology, severely impaired ability form oospores sporangia. addition, exhibited reduced pathogenicity compared wild type. 3,3-Diaminobenzidine (DAB) staining vitro hydrogen peroxide tolerance assays showed scavenging reactive oxygen species these significantly impaired. Taken together, results indicate regulates multiple processes P .