VdOGDH is involved in energy metabolism and required for virulence of Verticillium dahliae.

作者: Xiaokang Li , Xiaofeng Su , Guoqing Lu , Guoqing Sun , Zhuo Zhang

DOI: 10.1007/S00294-019-01025-2

关键词: Verticillium wiltCarbon utilizationVirulenceMicrobiologyMutantBiologyWild typeOGDHHyphaVerticillium dahliae

摘要: Verticillium dahliae, a soil-borne fungus, can invade plant vascular tissue and cause wilt. The enzyme α-oxoglutarate dehydrogenase (OGDH), catalyzing the oxidation of in tricarboxylic acid cycle (TCA), is vital for energy metabolism fungi. Here, we identified OGDH gene V. dahliae (VdOGDH, VDAG_10018) investigated its function virulence by generating deletion mutants (ΔVdOGDH) complementary (ΔVdOGDH-C). When ΔVdOGDH were supplemented with different carbon sources, vegetative growth on Czapek Dox medium was significantly impaired, suggesting that VdOGDH crucial utilization. Conidia atypically rounded or spherical, hyphae irregularly branched lacked typical whorled branches. Mutants ΔVdOGDH-1 ΔVdOGDH-2 highly sensitive to H2O2 plates had higher intracellular ROS levels. also elevated expression oxidative response-related genes, indicating involved response stress. In addition, disruption caused significant increase metabolism-related genes VdICL, VdICDH, VdMDH, VdPDH melanin-related Vayg1, VdSCD, VdLAC, VT4HR, VaflM mutants; thus, important melanin accumulation. Cotton plants inoculated exhibited mild leaf chlorosis disease index lower compared wild type ΔVdOGDH-C strains. These results together show essential full regulating multiple fungal developmental factors.

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