Determination and pharmacokinetics of gastrodin in human plasma by HPLC coupled with photodiode array detector.

摘要: Abstract In present study, an HPLC method coupled with photodiode array detector (HPLC-PDA) was established for determination and pharmacokinetics of gastrodin (GAS) in human plasma after oral administration GAS capsule. the method, ethanol dichloromethane were respectively used deproteinization purification during sample preparation procedure. Separation achieved on AichromBond-AQ C18 column (5 μm, 150 mm × 4.6 mm) mobile phase methanol–0.1% phosphoric acid solution (2:98, v/v) at a flow rate 0.8 ml/min. The wavelength set 220 nm injection volume 20 μl. Under conditions, calibration curve linear within concentration range 50–4000 ng/ml correlation coefficient (r) 0.99554 (weight = 1/X2) lower limit quantification (LLOQ) 50 ng/ml. inter- intra-day precisions less than 11% accuracies (%) 95.55–103.78%. extraction recoveries over 65% RSDs 5.50%. proved to be stable under tested conditions. Thus, valid enough applied pharmacokinetic study plasma. parameters 200 mg capsule described as: Cmax, 1484.55 ± 285.05 ng/ml; Tmax, 0.81 ± 0.16 h; t1/2α, 3.78 ± 2.33 h; t1/2β, 6.06 ± 3.20 h; t1/2Ka, 0.18 ± 0.53 h; K12, 0.18 ± 0.41/h; K21, 0.20 ± 0.16/h; K10, 4.11 ± 15.81/h; V1/F, 180.35 ± 89.44 L; CL/F, 62.50 ± 140.03 l/h; AUC0→t, 5619.41 ± 1972.88 (ng/ml) h; AUC0→∞, 7210.26 ± 3472.74 (ng/ml) h, respectively. These will useful clinical application GAS.