12-O-Tetradecanoylphorbol-13-acetate (TPA) directly inhibits spontaneous immunoglobulin secretion by in vivo antigen-induced human lymphoblastoid B cells.

摘要: Tetanus toxoid (Tet) booster immunization induces the transient appearance in circulation of lymphoblastoid (LB) B cells which spontaneously produce anti-tetanus IgG antibody (IgG-Tet) during a 3-dayin vitro culture. In this study we have examined effects TPA on ability LB to secrete and found that as little 10 ng/ml provoked marked inhibition induced cells' IgG-Tet production. This inhibitory effect was observed only when added early culture could be achieved by pretreating with for 1 hr. Only marginal production if addition delayed 14–24 The not mediated contaminant T increasing numbers cell cultures proportionally reversed effect. Likewise, synthesis due monocyte-dependent mechanism since (1) substantial depletion adherent did reverse synthesis, (2) monocyte-enriched population monocyte-depleted enhance, but fact partially reversed, caused TPA, (3) monocyte populations pretreated B-cell fractions inhibit subsequent cells.