Effect of ejaculate, bull, and a double swim-up sperm processing method on sperm sex ratio.
作者： Ninoska Madrid-Bury , Raúl Fernández , Adela Jiménez , Sonia Pérez-Garnelo , Pedro Nuno Moreira
DOI: 10.1017/S0967199403002272
关键词:
摘要: Offspring gender preselection has applications of considerable economic, health and ecological interest. In this study we analysed modifications the percentages spermatozoa bearing Y X chromosomes when semen samples are submitted to a double swim-up technique as possible method for producing embryos known sex with in vitro fertilisation protocols. As an initial experiment provide accurate evaluation determined incidence natural deviations primary ratio between bulls or ejaculates, analysing percentage Y-chromosome DNA (%Y-CDBS) polymerase chain reaction (PCR) amplification X- Y-specific fragments. Ejaculates were tested by direct semiquantitative PCR sexing blastocysts produced these spermatozoa. Bulls ejaculates did not have any effect on %Y-CDBS using ejaculates. However, our sperm preparation differences some fractions, suggesting that there intrinsic capacitation Y-bearing might be used produce desired fertilisation.
sci-hub.se 参考文章(21)
Edward E. Wallach, Benjamin E. Reubinoff, Joseph G. Schenker, New advances in sex preselection Fertility and Sterility. ,vol. 66, pp. 343- 350 ,(1996) , 10.1016/S0015-0282(16)58498-2
Susan M. Lobel, Robert J. Pomponio, George L. Mutter, The sex ratio of normal and manipulated human sperm quantitated by the polymerase chain reaction Fertility and Sterility. ,vol. 59, pp. 387- 392 ,(1993) , 10.1016/S0015-0282(16)55682-9
JOHN J. PARRISH, ROBERT H. FOOTE, Quantification of Bovine Sperm Separation by a Swim-up Method Relationship to Sperm Motility, Integrity of Acrosomes, Sperm Migration in Polyacrylamide Gel and Fertility Journal of Andrology. ,vol. 8, pp. 259- 266 ,(1987) , 10.1002/J.1939-4640.1987.TB03319.X
J.H. Check, D. Katsoff, A prospective study to evaluate the efficacy of modified swim-up preparation for male sex selection Human Reproduction. ,vol. 8, pp. 211- 214 ,(1993) , 10.1093/OXFORDJOURNALS.HUMREP.A138024
ML Checa, S Dunner, J Canon, None, Prediction of X and Y chromosome content in bovine sperm by using DNA pools through capillary electrophoresis Theriogenology. ,vol. 58, pp. 1579- 1586 ,(2002) , 10.1016/S0093-691X(02)01077-4
B.R. Bahnak, Q.Y. Wu, L. Coulombel, L. Drouet, D. Kerbiriou-Nabias, D. Meyer, A simple and efficient method for isolating high molecular weight DNA from mammalian sperm Nucleic Acids Research. ,vol. 16, pp. 1208- 1208 ,(1988) , 10.1093/NAR/16.3.1208
R. J. ERICSSON, C. N. LANGEVIN, M. NISHINO, Isolation of fractions rich in human Y sperm. Nature. ,vol. 246, pp. 421- 424 ,(1973) , 10.1038/246421A0
Martina Guttenbach, Wolfgang Engel, M. Schmid, Analysis of structural and numerical chromosome abnormalities in sperm of normal men and carriers of constitutional chromosome aberrations. A review. Human Genetics. ,vol. 100, pp. 1- 21 ,(1997) , 10.1007/S004390050459
B. Brandriff, L. Gordon, L. Ashworth, G. Watchmaker, D. Moore, A. J. Wyrobek, A. V. Carrano, Chromosomes of human sperm: variability among normal individuals. Human Genetics. ,vol. 70, pp. 18- 24 ,(1985) , 10.1007/BF00389451
L.M. Penfold, C. Holt, W.V. Holt, G.R. Welch, D.G. Cran, L.A. Johnson, Comparative motility of X and Y chromosome–bearing bovine sperm separated on the basis of DNA content by flow sorting Molecular Reproduction and Development. ,vol. 50, pp. 323- 327 ,(1998) , 10.1002/(SICI)1098-2795(199807)50:3<323::AID-MRD8>3.0.CO;2-L