An Efficient System to Establish Biopsy-Derived Trophoblastic Cell Lines from Bovine Embryos

摘要: Trophoblastic cells play a crucial role in implantation and placentogenesis can be used as model to provide substantial information on the peri-implantation period. Unfortunately, there are few cell lines for this purpose cattle because of difficulty raising successive stocks long-term. Our results show that combination monolayer culture system microdrops surface treated with gelatin employment conditioned media from mouse embryonic fibroblasts support growth bovine trophoblastic an embryo biopsy. Expression profiles mononucleate- binucleate-specific genes established represented various stages gestation. Moreover, ability expand more than 2 yr together pluripotency-related gene expression patterns revealed certain self-renewal capacity. In summary, we have developed vitro biopsy solves limitations using amplified DNA small number genotyping epigenotyping and, other hand, facilitates establishment useful models.