CDX2 regulates multiple trophoblast genes in bovine trophectoderm CT‐1 cells

摘要: The bovine trophectoderm (TE) undergoes a dramatic morphogenetic transition prior to uterine endometrial attachment. Many studies have documented trophoblast-specific gene expression profiles at various pre-attachment stages, yet genetic interactions within the transitioning TE regulatory network are not well characterized. During embryogenesis, transcription factors OCT4 and CDX2 co-expressed during early trophoblast elongation. In this study, trophectoderm-derived CT-1 cell line was utilized as model examine roles of network. An RT-PCR screen for TE-lineage identified CDX2, ERRB, ID2, SOX15, ELF5, HAND1, ASCL2. cells also express nuclear-localized, 360 amino acid ortholog pluripotency-specific human OCT4A. To delineate network, levels were manipulated via overexpression siRNA-mediated knockdown. increase in negatively regulated expression, but increased IFNT, ASCL2, ELF5. A reduction exhibited reciprocal effect, resulting decreased SOX15. Both knockdown ETS2 transcription. contrast manipulation only revealed positive autoregulatory mechanism upregulation Together, these results suggest that is core regulator multiple genes cells.