Establishment of Trophectoderm Cell Lines from Buffalo (Bubalus bubalis) Embryos of Different Sources and Examination of In Vitro Developmental Competence, Quality, Epigenetic Status and Gene Expression in Cloned Embryos Derived from Them

摘要: Despite being successfully used to produce live offspring in many species, somatic cell nuclear transfer (NT) has had a limited applicability due very low (>1%) birth rate because of high incidence pregnancy failure, which is mainly placental dysfunction. Since this may be abnormalities the trophectoderm (TE) lineage, TE cells can model understand growth disorders seen after NT. We isolated and characterized buffalo from blastocysts produced by vitro fertilization (TE-IVF) Hand-made cloning (TE-HMC), compared their characteristics gene expression, developed feeder-free culture system for long-term culture. The TE-IVF were then as donor HMC embryos following developmental competence, quality, epigenetic status expression with those using fetal or adult fibroblasts cells. found that although TE-HMC have similar capability grow culture, significant differences exist levels between them IVF they are derived, role associated NT pregnancies. Although producing blastocysts, competence quality lower than fibroblasts. level important genes different IVF.