Isolation and characterization of the trophectoderm from the Arabian camel (Camelus dromedarius)
作者： Islam M. Saadeldin , Ayman Abdel-Aziz Swelum , Mona Elsafadi , Abdullah F. Moumen , Faisal A. Alzahrani
DOI: 10.1016/J.PLACENTA.2017.06.015
关键词:
摘要: Abstract We isolated and characterized trophoblast from in vivo-derived camel embryos compared with embryonic stem-like cells. Camel were flushed on day 8 post-insemination used to derive trophectoderm cells under feeder-free culture conditions using a basement membrane matrix. Embryos evaluated for the expression of POU5F1, MYC, KLF4, SOX2, CDX2, KRT8 mRNA transcripts by relative quantitative polymerase chain reaction. grew expanded ∼4.5 mm maintained their vesicular shape in vitro 21 days post-insemination. Trophoblast cell lines showed distinct morphological criteria normal chromosomal counts. Embryonic positive staining in alkaline phosphatase significant increase KRT8, SOX2 whole embryos. decrease CDX2 expression. POU5F1 MYC no difference between both lines. embryo survival in vitro, particularly derivation cells, providing foundation further analysis early development placentation camels.
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