Quercetin ameliorates oxidative stress, inflammation and apoptosis in the heart of streptozotocin-nicotinamide-induced adult male diabetic rats

摘要: Abstract Introduction Quercetin is known to possess beneficial effects in ameliorating diabetic complications, however the mechanisms underlying cardioprotective effect of this compound diabetes not fully revealed. In study, quercetin on oxidative stress, inflammation and apoptosis heart were investigated. Normal streptozotocin-nicotinamide induced adult male rats received (10, 25 50 mg/kg/bw) orally for 28 days anesthetized hemodynamic parameters i.e. systolic blood pressure (SBP), diastolic (DBP) rate (HR) measured. Blood was collected analyses fasting glucose (FBG), insulin cardiac injury marker levels (troponin-C, CK-MB LDH). Following sacrificed, harvested histopathological changes observed. Heart subjected stress lipid peroxidation activity expression anti-oxidative enzymes SOD, CAT GPx. Levels determined by measuring nuclear factor (p65-NF-κB), tumor necrosis (TNF-α), interleukins (IL)-1β IL-6 using enzyme-linked immunoassay (ELISA). Distribution TNF-α Ikk-β (inflammatory markers), caspase-3, caspase-9, Blc-2 Bax (apoptosis markers) identified immunohistochemistry Western blotting respectively. Results Administration caused significant decrease FBG with increased levels. rat heart, lesser observed markedly decreased. Conclusions could potentially be used ameliorate myocardial damage due diabetes.