Prevalence of genotypic and phenotypic resistance to anti-retroviral drugs in a cohort of therapy-naïve HIV-1 infected US military personnel.

摘要: OBJECTIVE While transmission of drug-resistant HIV-1 has been reported, estimates prevalence resistance in drug-naive populations are incomplete. We investigated the genotypic mutations and phenotypic antiretroviral a cohort infected U.S. military personnel prior to institution therapy. DESIGN Cross-sectional study. METHODS Plasma was obtained from 114 recently subjects enrolled an epidemiological Genotypic determined by consensus sequencing PCR product pol gene. Sequences were interpreted phenotypic-genotypic correlative database. Resistance phenotypes recombinant virus cell culture assay. RESULTS found at higher than expected frequency. non-nucleoside reverse transcriptase inhibitors most common, with 15% 95 genotype 26% 91 phenotype. respectively 4% 8% for nucleoside 10% 1% protease inhibitors. One subject harbored all three drug classes. CONCLUSIONS A substantial frequency drugs identified therapy-naive cohort. In cases, assays yielded similar results, although assay could detect some inhibitor resistance-associated absence resistance. These data suggest need optimization treatment guidelines based on current seroconverters.