Biologically active components from mycobacterial cell walls. V. Granuloma formation in mouse lungs and guinea pig skin.

摘要: Abstract The ability of certain mycobacterial cell wall components, when attached to minute droplets oil, induce granulomatous inflammation in mouse lung and guinea pig skin has been studied for clues the mechanisms by which these components protect mice against aerosol challenge with virulent tubercle bacilli are useful immunotherapy dermal tumors pigs. Fractions examined were following: skeleton (CWS-I), consisted a polymeric peptidoglycolipid; wax D, contained mono- or oligomeric trehalose mycolates (P3). A combination CWS-I P3 induced more granuloma lungs than either two entities alone. Similarly, D was separated into chloroform-methanol-insoluble portion (CMI), peptidoglycolipid, soluble (CMS), P3, it found that both moieties required produce maximal inflammation. degree nonliving vaccines, as measured an increase weight following intravenous inoculation, could be correlated protection produced airborne infection M. tuberculosis H37Rv regression pigs upon intralesional inoculation. Even though quantitation response is difficult, would appear intradermal inoculation walls, CWS-I, CMI comparable granuloma, whereas CMS alone essentially inactive.