Development of novel extraction and separation methods for the determination of anthracyclines and taxanes simultaneously from biological matrices

摘要: For certain types of advanced cancers patients may be given a combination an anthracycline and taxane for chemotherapy treatment. Despite the fact that mixture anthracyclines taxanes administered simultaneously to accurate measurement drug levels during is proven more beneficial patients, currently no chromatographic method exists any drugs in single assay. It would useful carry out therapeutic monitoring (TDM) order assess how patient metabolising see whether dosage working or should altered. Such information therapy can very important clinical tool oncologist, enabling them change even regimen on individual basis improving outcome long run. This thesis describes development two different analytical methods capable quantifying both The first employed use on-line SPE-LC-UV this was then transferred mass spectrometric detector sensitive drugs. SPE-LC-MS assay did result lower limits detection fully automated. entire extraction, separation achieved by column-switching between SPE column column. optimum type switching valve, extraction conditions separating were evaluated obtain highest recovery possible each target analyte human serum. Recoveries ranged from 86 117% LC-UV 95 113% most analytes LC-MS This research could potentially lead introduction Ireland cancer being treated with both.